rabbit anti mouse tom20 Search Results


dshb 1d4b rabbit anti tomm20  (Developmental Studies Hybridoma Bank)
99
Developmental Studies Hybridoma Bank dshb 1d4b rabbit anti tomm20
Dshb 1d4b Rabbit Anti Tomm20, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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antibodies rabbit tom 20  (Proteintech)
96
Proteintech antibodies rabbit tom 20
Antibodies Rabbit Tom 20, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
antibodies rabbit tom 20 - by Bioz Stars, 2026-03
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membrane 20  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology membrane 20
Membrane 20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/membrane 20/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
membrane 20 - by Bioz Stars, 2026-03
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42406 cgas mouse  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc 42406 cgas mouse
FIGURE 4. NR mitigated damaged DNA-stimulated <t>cGAS-STING</t> pathway. (A) Representative microscopic images of dsDNA immunopositivity (in red) and DAPI-counterstained nuclei (in blue). Scale bar: 50 μm. The boxed areas within the images were magnified and displayed in the right panels (arrowheads). Scale bar: 10 μm. (B) Colocalization of Tom20 (green), dsDNA (red), and DAPI (blue) expressions in retinas of each group (arrows). Scale bar: 10 μm. (C) Heatmap of cGAS and STING from RNA sequencing of naive and LIRD retinas with or without NR treatment (n = 4/group). (D) Colocalization of cGAS (red) and Iba1-positive microglia (green) expression in the retinas of each group (arrows). Scale bar: 30 μm. (E) Quantification of cGAS-positive microglia of each group in the ONL at three days after light exposure (n = 4–6/group). (F) Colocalization of STING (red) and Iba1-positive microglia (green) expression in the retinas of each group (arrows). Scale bar: 30 μm. (G) Quantification of STING-positive microglia of each group in the ONL at three days after light exposure (n = 5–6/group). NR treatment significantly decreased the expression of cGAS and STING compared with the PBS-treated group, ****P < 0.0001.
42406 Cgas Mouse, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/42406 cgas mouse/product/Cell Signaling Technology Inc
Average 99 stars, based on 1 article reviews
42406 cgas mouse - by Bioz Stars, 2026-03
99/100 stars
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memerald tomm20 n 10  (Addgene inc)
92
Addgene inc memerald tomm20 n 10
FIGURE 4. NR mitigated damaged DNA-stimulated <t>cGAS-STING</t> pathway. (A) Representative microscopic images of dsDNA immunopositivity (in red) and DAPI-counterstained nuclei (in blue). Scale bar: 50 μm. The boxed areas within the images were magnified and displayed in the right panels (arrowheads). Scale bar: 10 μm. (B) Colocalization of Tom20 (green), dsDNA (red), and DAPI (blue) expressions in retinas of each group (arrows). Scale bar: 10 μm. (C) Heatmap of cGAS and STING from RNA sequencing of naive and LIRD retinas with or without NR treatment (n = 4/group). (D) Colocalization of cGAS (red) and Iba1-positive microglia (green) expression in the retinas of each group (arrows). Scale bar: 30 μm. (E) Quantification of cGAS-positive microglia of each group in the ONL at three days after light exposure (n = 4–6/group). (F) Colocalization of STING (red) and Iba1-positive microglia (green) expression in the retinas of each group (arrows). Scale bar: 30 μm. (G) Quantification of STING-positive microglia of each group in the ONL at three days after light exposure (n = 5–6/group). NR treatment significantly decreased the expression of cGAS and STING compared with the PBS-treated group, ****P < 0.0001.
Memerald Tomm20 N 10, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/memerald tomm20 n 10/product/Addgene inc
Average 92 stars, based on 1 article reviews
memerald tomm20 n 10 - by Bioz Stars, 2026-03
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mouse rabbit mab cell signaling 2679 ab 2228381 tom20  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc mouse rabbit mab cell signaling 2679 ab 2228381 tom20
Characteristics of antibodies used for Western blotting and microscopy
Mouse Rabbit Mab Cell Signaling 2679 Ab 2228381 Tom20, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse rabbit mab cell signaling 2679 ab 2228381 tom20/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
mouse rabbit mab cell signaling 2679 ab 2228381 tom20 - by Bioz Stars, 2026-03
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rabbit monoclonal anti tom20 d8t4n antibody  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc rabbit monoclonal anti tom20 d8t4n antibody
TLE inhibits glutamate-induced excessive mitophagy. HT-22 cells (passage 14,16,17) were pretreated with 50 µg/mL of TLE or 100 nM selenium followed by 5 mM glutamate for 18 h. ( a ) The protein expression level of LC3B (autophagy) and <t>TOM20</t> (mitochondria) were analyzed by Western blot, and β-actin served as the loading control. Relative protein levels of ( b ) LC3B and ( c ) TOM20 were quantified by densitometry and the mean data from at least three independent experiments were normalized to the results. Cont, untreated control; Starv, starvation. The data represent the means ± SEM. * p value < 0.05, ** p value < 0.01, *** p value < 0.005 compared with untreated control # p value < 0.05, ## p value < 0.01 compared with only the glutamate-treated group.
Rabbit Monoclonal Anti Tom20 D8t4n Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit monoclonal anti tom20 d8t4n antibody/product/Cell Signaling Technology Inc
Average 94 stars, based on 1 article reviews
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mouse anti tom20  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology mouse anti tom20
TLE inhibits glutamate-induced excessive mitophagy. HT-22 cells (passage 14,16,17) were pretreated with 50 µg/mL of TLE or 100 nM selenium followed by 5 mM glutamate for 18 h. ( a ) The protein expression level of LC3B (autophagy) and <t>TOM20</t> (mitochondria) were analyzed by Western blot, and β-actin served as the loading control. Relative protein levels of ( b ) LC3B and ( c ) TOM20 were quantified by densitometry and the mean data from at least three independent experiments were normalized to the results. Cont, untreated control; Starv, starvation. The data represent the means ± SEM. * p value < 0.05, ** p value < 0.01, *** p value < 0.005 compared with untreated control # p value < 0.05, ## p value < 0.01 compared with only the glutamate-treated group.
Mouse Anti Tom20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti tom20/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
mouse anti tom20 - by Bioz Stars, 2026-03
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rabbit polyclonal anti tom20  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology rabbit polyclonal anti tom20
TLE inhibits glutamate-induced excessive mitophagy. HT-22 cells (passage 14,16,17) were pretreated with 50 µg/mL of TLE or 100 nM selenium followed by 5 mM glutamate for 18 h. ( a ) The protein expression level of LC3B (autophagy) and <t>TOM20</t> (mitochondria) were analyzed by Western blot, and β-actin served as the loading control. Relative protein levels of ( b ) LC3B and ( c ) TOM20 were quantified by densitometry and the mean data from at least three independent experiments were normalized to the results. Cont, untreated control; Starv, starvation. The data represent the means ± SEM. * p value < 0.05, ** p value < 0.01, *** p value < 0.005 compared with untreated control # p value < 0.05, ## p value < 0.01 compared with only the glutamate-treated group.
Rabbit Polyclonal Anti Tom20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti tom20/product/Santa Cruz Biotechnology
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rabbit anti tom20 ab  (OriGene)
92
OriGene rabbit anti tom20 ab
TLE inhibits glutamate-induced excessive mitophagy. HT-22 cells (passage 14,16,17) were pretreated with 50 µg/mL of TLE or 100 nM selenium followed by 5 mM glutamate for 18 h. ( a ) The protein expression level of LC3B (autophagy) and <t>TOM20</t> (mitochondria) were analyzed by Western blot, and β-actin served as the loading control. Relative protein levels of ( b ) LC3B and ( c ) TOM20 were quantified by densitometry and the mean data from at least three independent experiments were normalized to the results. Cont, untreated control; Starv, starvation. The data represent the means ± SEM. * p value < 0.05, ** p value < 0.01, *** p value < 0.005 compared with untreated control # p value < 0.05, ## p value < 0.01 compared with only the glutamate-treated group.
Rabbit Anti Tom20 Ab, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
rabbit anti tom20 ab - by Bioz Stars, 2026-03
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tom20  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology tom20
TLE inhibits glutamate-induced excessive mitophagy. HT-22 cells (passage 14,16,17) were pretreated with 50 µg/mL of TLE or 100 nM selenium followed by 5 mM glutamate for 18 h. ( a ) The protein expression level of LC3B (autophagy) and <t>TOM20</t> (mitochondria) were analyzed by Western blot, and β-actin served as the loading control. Relative protein levels of ( b ) LC3B and ( c ) TOM20 were quantified by densitometry and the mean data from at least three independent experiments were normalized to the results. Cont, untreated control; Starv, starvation. The data represent the means ± SEM. * p value < 0.05, ** p value < 0.01, *** p value < 0.005 compared with untreated control # p value < 0.05, ## p value < 0.01 compared with only the glutamate-treated group.
Tom20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tom20/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
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anti tom20  (Bio-Rad)
90
Bio-Rad anti tom20
TLE inhibits glutamate-induced excessive mitophagy. HT-22 cells (passage 14,16,17) were pretreated with 50 µg/mL of TLE or 100 nM selenium followed by 5 mM glutamate for 18 h. ( a ) The protein expression level of LC3B (autophagy) and <t>TOM20</t> (mitochondria) were analyzed by Western blot, and β-actin served as the loading control. Relative protein levels of ( b ) LC3B and ( c ) TOM20 were quantified by densitometry and the mean data from at least three independent experiments were normalized to the results. Cont, untreated control; Starv, starvation. The data represent the means ± SEM. * p value < 0.05, ** p value < 0.01, *** p value < 0.005 compared with untreated control # p value < 0.05, ## p value < 0.01 compared with only the glutamate-treated group.
Anti Tom20, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 4. NR mitigated damaged DNA-stimulated cGAS-STING pathway. (A) Representative microscopic images of dsDNA immunopositivity (in red) and DAPI-counterstained nuclei (in blue). Scale bar: 50 μm. The boxed areas within the images were magnified and displayed in the right panels (arrowheads). Scale bar: 10 μm. (B) Colocalization of Tom20 (green), dsDNA (red), and DAPI (blue) expressions in retinas of each group (arrows). Scale bar: 10 μm. (C) Heatmap of cGAS and STING from RNA sequencing of naive and LIRD retinas with or without NR treatment (n = 4/group). (D) Colocalization of cGAS (red) and Iba1-positive microglia (green) expression in the retinas of each group (arrows). Scale bar: 30 μm. (E) Quantification of cGAS-positive microglia of each group in the ONL at three days after light exposure (n = 4–6/group). (F) Colocalization of STING (red) and Iba1-positive microglia (green) expression in the retinas of each group (arrows). Scale bar: 30 μm. (G) Quantification of STING-positive microglia of each group in the ONL at three days after light exposure (n = 5–6/group). NR treatment significantly decreased the expression of cGAS and STING compared with the PBS-treated group, ****P < 0.0001.

Journal: Investigative ophthalmology & visual science

Article Title: Nicotinamide Riboside Mitigates Retinal Degeneration by Suppressing Damaged DNA-Stimulated Microglial Activation and STING-Mediated Pyroptosis.

doi: 10.1167/iovs.66.4.14

Figure Lengend Snippet: FIGURE 4. NR mitigated damaged DNA-stimulated cGAS-STING pathway. (A) Representative microscopic images of dsDNA immunopositivity (in red) and DAPI-counterstained nuclei (in blue). Scale bar: 50 μm. The boxed areas within the images were magnified and displayed in the right panels (arrowheads). Scale bar: 10 μm. (B) Colocalization of Tom20 (green), dsDNA (red), and DAPI (blue) expressions in retinas of each group (arrows). Scale bar: 10 μm. (C) Heatmap of cGAS and STING from RNA sequencing of naive and LIRD retinas with or without NR treatment (n = 4/group). (D) Colocalization of cGAS (red) and Iba1-positive microglia (green) expression in the retinas of each group (arrows). Scale bar: 30 μm. (E) Quantification of cGAS-positive microglia of each group in the ONL at three days after light exposure (n = 4–6/group). (F) Colocalization of STING (red) and Iba1-positive microglia (green) expression in the retinas of each group (arrows). Scale bar: 30 μm. (G) Quantification of STING-positive microglia of each group in the ONL at three days after light exposure (n = 5–6/group). NR treatment significantly decreased the expression of cGAS and STING compared with the PBS-treated group, ****P < 0.0001.

Article Snippet: Iba-1 Rabbit 1:500 Abcam* ab178846 Iba-1 Goat 1:800 Abcam* ab5076 CD68 (FA-11) Rat 1:200 Thermo† 14-0681-82 dsDNA Mouse 1:200 Santa Cruz‡ sc-58749 Tom20 (D8T4N) Rabbit 1:200 Cell Signaling Technology§ 42406 Cgas Mouse 1:200 Santa Cruz‡ sc-515777 STing Rabbit 1:200 Proteintech║ 19851-1-AP NLRP3 Rabbit 1:200 Abmart¶ P60622R3 Casepase-1 Mouse 1:100 Santa Cruz‡ sc-56036 IL-1β Mouse 1:100 Cell Signaling Technology§ 12242 GSDMD Mouse 1:200 Santa Cruz† sc-393581 Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) Goat 1:400 Jackson# 111-545-003 Goat Anti-Rat IgG H&L (Alexa Fluor 594) Goat 1:300 Jackson# 112-505-175 Donkey Anti-Rabbit IgG H&L (Alexa Fluor 488) Donkey 1:400 Thermo† A21206 Donkey Anti-Mouse IgG H&L (Alexa Fluor 555) Donkey 1:400 Thermo† A31570 Donkey Anti-goat IgG H&L (Alexa Fluor 568) Donkey 1:400 Thermo† A11057 * Abcam, Cambridge, MA, USA.

Techniques: RNA Sequencing, Expressing

Characteristics of antibodies used for Western blotting and microscopy

Journal: Endocrinology

Article Title: Luteal Lipid Droplets: A Novel Platform for Steroid Synthesis

doi: 10.1210/endocr/bqad124

Figure Lengend Snippet: Characteristics of antibodies used for Western blotting and microscopy

Article Snippet: Antibody name Dilution ratio Species specificity Source Supplier (distributor) Catalog No RRID PLIN 2 1:1000 Bovine Guinea pig pAB Fitzgerald Industries International (Acton) 20R-AP002 AB_1282475 PLIN 3 1:1000 Bovine Guinea pig pAB Fitzgerald Industries International 20R-TP001 AB_1289040 STAR 1:10000 Mouse Rabbit pAB Abcam ab96637 AB_10678397 CYP11A1 1:1000 Mouse Rabbit mAB Cell Signaling 14217 AB_2631970 HSD3B 1:1000 a /1:200 b Bovine Mouse mAB Thermo Fisher MA1-46438 AB_2120102 VIM 1:1000 Bovine Rabbit pAB Abcam ab137321 AB_2921312 HSL 1:1000 Mouse Rabbit pAB Cell Signaling 4107 AB_2296900 COX IV 1:1000 Bovine Rabbit mAB Cell Signaling 4850 AB_2085424 HSP47 1:1000 Bovine Mouse mAB Enzo Life Sciences Inc SPA-470 AB_2185189 TOM70 1:1000 Mouse Mouse pAB Abcam ab89624 AB_2043080 CNX 1:200 Mouse Rabbit mAB Cell Signaling 2679 AB_2228381 TOM20 1:200 c Mouse Rabbit mAB Cell Signaling 42406S AB_2687663 TUBB 1:5000 Bovine Mouse mAB Sigma Life Science T4026 AB_477577 ACTB 1:5000 Bovine Mouse mAB Sigma Life Science A5441 AB_476744 Lipi-Blue 1 μM Dojindo Molecular LD01 BODIPY 493/503 10 μM Thermo Fisher D3922 MitoTracker Red FM 200nM Thermo Fisher M22425 HRP-linked 1:10000 Anti-guinea pig Jackson ImmunoResearch 106-035-003 AB_2337402 HRP-linked 1:10000 Anti-rabbit Jackson ImmunoResearch 111-035-003 AB_2313567 HRP-linked 1:10000 Anti-mouse Jackson ImmunoResearch 115-035-205 AB_10015289 DyLight 405 1:500 Anti-mouse Jackson ImmunoResearch 115-475-166 AB_2338786 Alexa Fluor 488 1:500 Anti-mouse Invitrogen A32723 AB_2633275 Alexa Fluor 594 1:500 Anti-rabbit Invitrogen A-11012 AB_2534079 Alexa Fluor 647 1:500 Anti-biotin BioLegend 405237 AB_2941953 Open in a separate window Abbreviations: ACTB, beta-actin (loading control); CNX, calnexin; COX IV, cytochrome c oxidase subunit 4; CYP11A1, cholesterol side-chain cleavage enzyme; HRP, horseradish peroxidase; HSD3B, 3 beta-hydroxysteroid dehydrogenase); HSL, hormone sensitive lipase; HSP47, heat shock protein 47; PLIN2, perilipin 2; PLIN3, perilipin 3; STAR, steroidogenic acute regulatory protein; TOM20, mitochondrial import receptor subunit 20; TOM70, mitochondrial import receptor subunit 70; TUBB, beta-tubulin (loading control); VIM, vimentin. a Dilution used for Western blotting. b Dilution used for confocal microscopy. c Biotinylated antibody.

Techniques: Western Blot

Subcellular localization of 3beta-hydroxysteroid dehydrogenase (HSD3B) in steroidogenic small luteal cells. Populations of small luteal cells were enriched from bovine luteal tissue and visualized using confocal microscopy. A, Representative confocal micrograph of HSD3B (red) and luteal lipid droplets (green), calnexin (endoplasmic reticulum; green), or TOM20 (mitochondria; green). White boxes represent an area of 10× enlargement. White dashed lines represent the area of intensity overlap. B, Representative quantitative analysis of intensity overlap. Arrows represent spatial location of lipid droplet. TOM20 (streptavidin, Alexa Fluor 647); calnexin (Alexa Fluor 594); lipid droplet (BODIPY493/503), and HSD3B (DyLight 405). Micron bar represents 20 and 2 µm, respectively.

Journal: Endocrinology

Article Title: Luteal Lipid Droplets: A Novel Platform for Steroid Synthesis

doi: 10.1210/endocr/bqad124

Figure Lengend Snippet: Subcellular localization of 3beta-hydroxysteroid dehydrogenase (HSD3B) in steroidogenic small luteal cells. Populations of small luteal cells were enriched from bovine luteal tissue and visualized using confocal microscopy. A, Representative confocal micrograph of HSD3B (red) and luteal lipid droplets (green), calnexin (endoplasmic reticulum; green), or TOM20 (mitochondria; green). White boxes represent an area of 10× enlargement. White dashed lines represent the area of intensity overlap. B, Representative quantitative analysis of intensity overlap. Arrows represent spatial location of lipid droplet. TOM20 (streptavidin, Alexa Fluor 647); calnexin (Alexa Fluor 594); lipid droplet (BODIPY493/503), and HSD3B (DyLight 405). Micron bar represents 20 and 2 µm, respectively.

Article Snippet: Antibody name Dilution ratio Species specificity Source Supplier (distributor) Catalog No RRID PLIN 2 1:1000 Bovine Guinea pig pAB Fitzgerald Industries International (Acton) 20R-AP002 AB_1282475 PLIN 3 1:1000 Bovine Guinea pig pAB Fitzgerald Industries International 20R-TP001 AB_1289040 STAR 1:10000 Mouse Rabbit pAB Abcam ab96637 AB_10678397 CYP11A1 1:1000 Mouse Rabbit mAB Cell Signaling 14217 AB_2631970 HSD3B 1:1000 a /1:200 b Bovine Mouse mAB Thermo Fisher MA1-46438 AB_2120102 VIM 1:1000 Bovine Rabbit pAB Abcam ab137321 AB_2921312 HSL 1:1000 Mouse Rabbit pAB Cell Signaling 4107 AB_2296900 COX IV 1:1000 Bovine Rabbit mAB Cell Signaling 4850 AB_2085424 HSP47 1:1000 Bovine Mouse mAB Enzo Life Sciences Inc SPA-470 AB_2185189 TOM70 1:1000 Mouse Mouse pAB Abcam ab89624 AB_2043080 CNX 1:200 Mouse Rabbit mAB Cell Signaling 2679 AB_2228381 TOM20 1:200 c Mouse Rabbit mAB Cell Signaling 42406S AB_2687663 TUBB 1:5000 Bovine Mouse mAB Sigma Life Science T4026 AB_477577 ACTB 1:5000 Bovine Mouse mAB Sigma Life Science A5441 AB_476744 Lipi-Blue 1 μM Dojindo Molecular LD01 BODIPY 493/503 10 μM Thermo Fisher D3922 MitoTracker Red FM 200nM Thermo Fisher M22425 HRP-linked 1:10000 Anti-guinea pig Jackson ImmunoResearch 106-035-003 AB_2337402 HRP-linked 1:10000 Anti-rabbit Jackson ImmunoResearch 111-035-003 AB_2313567 HRP-linked 1:10000 Anti-mouse Jackson ImmunoResearch 115-035-205 AB_10015289 DyLight 405 1:500 Anti-mouse Jackson ImmunoResearch 115-475-166 AB_2338786 Alexa Fluor 488 1:500 Anti-mouse Invitrogen A32723 AB_2633275 Alexa Fluor 594 1:500 Anti-rabbit Invitrogen A-11012 AB_2534079 Alexa Fluor 647 1:500 Anti-biotin BioLegend 405237 AB_2941953 Open in a separate window Abbreviations: ACTB, beta-actin (loading control); CNX, calnexin; COX IV, cytochrome c oxidase subunit 4; CYP11A1, cholesterol side-chain cleavage enzyme; HRP, horseradish peroxidase; HSD3B, 3 beta-hydroxysteroid dehydrogenase); HSL, hormone sensitive lipase; HSP47, heat shock protein 47; PLIN2, perilipin 2; PLIN3, perilipin 3; STAR, steroidogenic acute regulatory protein; TOM20, mitochondrial import receptor subunit 20; TOM70, mitochondrial import receptor subunit 70; TUBB, beta-tubulin (loading control); VIM, vimentin. a Dilution used for Western blotting. b Dilution used for confocal microscopy. c Biotinylated antibody.

Techniques: Confocal Microscopy

Subcellular localization of 3beta-hydroxysteroid dehydrogenase (HSD3B) in steroidogenic large luteal cells. Enriched populations of large luteal cells from bovine luteal tissue were visualized using confocal microscopy. A, Representative confocal micrograph of HSD3B (red) and luteal lipid droplets (green), calnexin (endoplasmic reticulum; green), or TOM20 (mitochondria; green). White boxes represent an area of 10× enlargement. White dashed lines represent the area of intensity overlap. B, Representative quantitative analysis of intensity overlap. Arrows represent spatial location of lipid droplet. TOM20 (streptavidin, Alexa Fluor 647); calnexin (Alexa Fluor 594); lipid droplet (BODIPY493/503), and HSD3B (DyLight 405). Micron bar represents 20 and 2 µm, respectively.

Journal: Endocrinology

Article Title: Luteal Lipid Droplets: A Novel Platform for Steroid Synthesis

doi: 10.1210/endocr/bqad124

Figure Lengend Snippet: Subcellular localization of 3beta-hydroxysteroid dehydrogenase (HSD3B) in steroidogenic large luteal cells. Enriched populations of large luteal cells from bovine luteal tissue were visualized using confocal microscopy. A, Representative confocal micrograph of HSD3B (red) and luteal lipid droplets (green), calnexin (endoplasmic reticulum; green), or TOM20 (mitochondria; green). White boxes represent an area of 10× enlargement. White dashed lines represent the area of intensity overlap. B, Representative quantitative analysis of intensity overlap. Arrows represent spatial location of lipid droplet. TOM20 (streptavidin, Alexa Fluor 647); calnexin (Alexa Fluor 594); lipid droplet (BODIPY493/503), and HSD3B (DyLight 405). Micron bar represents 20 and 2 µm, respectively.

Article Snippet: Antibody name Dilution ratio Species specificity Source Supplier (distributor) Catalog No RRID PLIN 2 1:1000 Bovine Guinea pig pAB Fitzgerald Industries International (Acton) 20R-AP002 AB_1282475 PLIN 3 1:1000 Bovine Guinea pig pAB Fitzgerald Industries International 20R-TP001 AB_1289040 STAR 1:10000 Mouse Rabbit pAB Abcam ab96637 AB_10678397 CYP11A1 1:1000 Mouse Rabbit mAB Cell Signaling 14217 AB_2631970 HSD3B 1:1000 a /1:200 b Bovine Mouse mAB Thermo Fisher MA1-46438 AB_2120102 VIM 1:1000 Bovine Rabbit pAB Abcam ab137321 AB_2921312 HSL 1:1000 Mouse Rabbit pAB Cell Signaling 4107 AB_2296900 COX IV 1:1000 Bovine Rabbit mAB Cell Signaling 4850 AB_2085424 HSP47 1:1000 Bovine Mouse mAB Enzo Life Sciences Inc SPA-470 AB_2185189 TOM70 1:1000 Mouse Mouse pAB Abcam ab89624 AB_2043080 CNX 1:200 Mouse Rabbit mAB Cell Signaling 2679 AB_2228381 TOM20 1:200 c Mouse Rabbit mAB Cell Signaling 42406S AB_2687663 TUBB 1:5000 Bovine Mouse mAB Sigma Life Science T4026 AB_477577 ACTB 1:5000 Bovine Mouse mAB Sigma Life Science A5441 AB_476744 Lipi-Blue 1 μM Dojindo Molecular LD01 BODIPY 493/503 10 μM Thermo Fisher D3922 MitoTracker Red FM 200nM Thermo Fisher M22425 HRP-linked 1:10000 Anti-guinea pig Jackson ImmunoResearch 106-035-003 AB_2337402 HRP-linked 1:10000 Anti-rabbit Jackson ImmunoResearch 111-035-003 AB_2313567 HRP-linked 1:10000 Anti-mouse Jackson ImmunoResearch 115-035-205 AB_10015289 DyLight 405 1:500 Anti-mouse Jackson ImmunoResearch 115-475-166 AB_2338786 Alexa Fluor 488 1:500 Anti-mouse Invitrogen A32723 AB_2633275 Alexa Fluor 594 1:500 Anti-rabbit Invitrogen A-11012 AB_2534079 Alexa Fluor 647 1:500 Anti-biotin BioLegend 405237 AB_2941953 Open in a separate window Abbreviations: ACTB, beta-actin (loading control); CNX, calnexin; COX IV, cytochrome c oxidase subunit 4; CYP11A1, cholesterol side-chain cleavage enzyme; HRP, horseradish peroxidase; HSD3B, 3 beta-hydroxysteroid dehydrogenase); HSL, hormone sensitive lipase; HSP47, heat shock protein 47; PLIN2, perilipin 2; PLIN3, perilipin 3; STAR, steroidogenic acute regulatory protein; TOM20, mitochondrial import receptor subunit 20; TOM70, mitochondrial import receptor subunit 70; TUBB, beta-tubulin (loading control); VIM, vimentin. a Dilution used for Western blotting. b Dilution used for confocal microscopy. c Biotinylated antibody.

Techniques: Confocal Microscopy

TLE inhibits glutamate-induced excessive mitophagy. HT-22 cells (passage 14,16,17) were pretreated with 50 µg/mL of TLE or 100 nM selenium followed by 5 mM glutamate for 18 h. ( a ) The protein expression level of LC3B (autophagy) and TOM20 (mitochondria) were analyzed by Western blot, and β-actin served as the loading control. Relative protein levels of ( b ) LC3B and ( c ) TOM20 were quantified by densitometry and the mean data from at least three independent experiments were normalized to the results. Cont, untreated control; Starv, starvation. The data represent the means ± SEM. * p value < 0.05, ** p value < 0.01, *** p value < 0.005 compared with untreated control # p value < 0.05, ## p value < 0.01 compared with only the glutamate-treated group.

Journal: Antioxidants

Article Title: Thunbergia laurifolia Leaf Extract Inhibits Glutamate-Induced Neurotoxicity and Cell Death through Mitophagy Signaling

doi: 10.3390/antiox10111678

Figure Lengend Snippet: TLE inhibits glutamate-induced excessive mitophagy. HT-22 cells (passage 14,16,17) were pretreated with 50 µg/mL of TLE or 100 nM selenium followed by 5 mM glutamate for 18 h. ( a ) The protein expression level of LC3B (autophagy) and TOM20 (mitochondria) were analyzed by Western blot, and β-actin served as the loading control. Relative protein levels of ( b ) LC3B and ( c ) TOM20 were quantified by densitometry and the mean data from at least three independent experiments were normalized to the results. Cont, untreated control; Starv, starvation. The data represent the means ± SEM. * p value < 0.05, ** p value < 0.01, *** p value < 0.005 compared with untreated control # p value < 0.05, ## p value < 0.01 compared with only the glutamate-treated group.

Article Snippet: Alexa Fluor 488, carbonyl cyanide m-chlorophenylhydrazone (CCCP), mouse monoclonal anti-β-actin antibody, mouse monoclonal anti-rabbit IgG, HRP-linked antibody, rabbit polyclonal anti-LC3B antibody, rabbit monoclonal anti-TOM20 (D8T4N) antibody and tetramethylrhodamine ethyl ester (TMRE) were purchased from Cell Signaling Technology (Denvers, MA, USA).

Techniques: Expressing, Western Blot, Control